LOCUS       EY190187                 549 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02c03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_492174.1| Phosphoenolpyruvate carboxykinase (73.4
            kD) [Caenorhabditis elegans] pir|T24168 hypothetical protein
            R11A5.4 - Caenorhabditis elegans emb|CAB05600.1| Hypothetical
            protein R11A5.4 [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY190187
VERSION     EY190187.1  GI:159492502
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 895 was extended to value 896 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 768
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggagg gcagaacggt tatgcacgat ccgatggcaa tgcgtccgtt catgggctac
       61 aatttcggca agtacttgga gcactggtgc agcctgaagg agcagggtcg caaagtgccg
      121 cagatcttcc acgtcaactg gttccgcaaa tcgaccgatg gcaaattcct ctggccaggc
      181 tattgtgaga acatccgcgt tattgactgg attgtgcgtc gtctggaggg cgaacaaggg
      241 atcggagtgg acactgcaat cggaactgtt ccaactcgcg aatcaatgaa cctggagggg
      301 ctcggaaata tcaactggga acagctaatg tctctgccgg ctgactactg gcaggaagat
      361 gccaaggaag tccgcaagtt cttggaggca caggtgggat ctgatctgcc ggatgcagtt
      421 cgcaacgaga tggatgcaca ggaaaagcgg attgaggcgc tctgatccag aaagtgcaca
      481 agatattatt cgttcagcag aatattttcc ttgattataa tttaatcgtg acaacgatgt
      541 attattttt
//