LOCUS       EY190184                 492 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02b11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_492323.1| Putative membrane protein, with at
            least 2 transmembrane domains, of bilaterial origin (19.0 kD)
            [Caenorhabditis elegans] emb|CAA99892.2| Hypothetical protein
            R05D11.5 [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY190184
VERSION     EY190184.1  GI:159492659
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 851 was extended to value 851 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 160
            original value before phred recall for SR was 693
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa cgcaaccaag tggttgattc cgggctggat ctgaacgacc cgaatgcatt
       61 tggcgaatat tgcaaagacg tggtcattct gagtgtgatg gcacagctac tgagtcttct
      121 ctccagctat ttctacttgt tgctgctcat agttcccatg tttgcgctat tcaaactttg
      181 ggtcaaggta ctggctccgt ggatatttgc gccagcggaa gaatcggatg aaaaagatga
      241 cggaaaaaag aggcaaaaac gccagaagtt tgtgcatgtg aggcggtaaa tgtcaaaaac
      301 tgttgtaacc aactcactct ctgcaagatt agactttggg ccgaattcct caatttaatc
      361 taatttattt ttgtcttgtt aaatgtggac tggcccttca gttattctat ttgtttctaa
      421 ccgattccat cattcttcgt ttattgggca aaacaagtcc aaatatattt gcagttgtaa
      481 aacatgttta aa
//