LOCUS       EY190180                 596 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02b07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_510044.1| Kunitz/Bovine pancreatic trypsin
            inhibitor domain and EB module family member [Caenorhabditis
            elegans] pir|T20406 hypothetical protein E01G6.1 - Caenorhabditis
            elegans emb|CAA93531.1| Hypothetical protein E01G6.1
            [Caenorhabditis e, mRNA sequence.
ACCESSION   EY190180
VERSION     EY190180.1  GI:159492655
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 895 was extended to value 895 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 897
            COMM   possible reversed clone; protein similarity on positive
            strand
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc attactatta ttattgtcgg atgcgaattc taccgcaaag aatgcatttc
       61 caattggcct ttcatgttct gaatccgaag cctcatactc caaaagctct tcgtctgcag
      121 catgatctcg aagaagacgg ttggctgcgc ttccttggaa acgacttccc tccacctcca
      181 tgcaaatgcc ctaaaatcgg taaagaaaat ttgcttcaca aacatatttg ttctggcctt
      241 gtctgtcctg ttttgtaggt cgtcaatgcg cagttcataa ccaggacgac attggcaagt
      301 cagctaaaat cgaactttta gtaatattca atgtcccctg atcgtcatca ccgttctttc
      361 actgcaatac gaattgtcta ccatgcattg ctcttcagcc tgcaaattat aaatttattt
      421 cgaaaaattt tgatgaaagt cacaccacaa cggtgggaag gcagaatccg ccgatggtcc
      481 cattgtcgcg ttcgtccacc gaatccatga acacaatcga agtgaggtcg tgacaacggc
      541 cagcgatgtt caggaatccc tcgggacatt ggatgacgca tttggattca tgaatg
//