LOCUS       EY190176                 200 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02b03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190176
VERSION     EY190176.1  GI:159492651
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 200)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 895 was extended to value 895 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 160
            original value before phred recall for SR was 404
            PolyA tail found inside read...SR being truncated to 365
            Potential full insert read...vec-to-vec length: 337
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat gtgattgcgg cgtgcggttc ggctggcgaa ccagggggac tgcggcaagc
       61 gcaaagactg gatgaagacc aactgcaaga agtcctgcgg actctgttga gaaacagaat
      121 acttttggcc attatcgcga cattttttaa acttttaaag ccttagagat tgcgcttgta
      181 cctattaaaa agcgtgggga
//