LOCUS EY190167 455 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa02a04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_492680.1| Isoleucyl tRNA Synthetase IRS-2 (irs-2) [Caenorhabditis elegans] pir|T19442 hypothetical protein C25A1.7a - Caenorhabditis elegans emb|CAB02766.1| C. elegans IRS-2 protein (corresponding sequence C25A1.7a) [Caenorhabditis elegans], mRNA sequence. ACCESSION EY190167 VERSION EY190167.1 GI:159492642 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 455) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 152 original value before phred recall for SR was 903 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..455 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggcg gctgaagcaa attcggctca ttcttcgctt cattcttggc gcattggaat 61 cggcggccaa cgcaaagctg gcgactggga gagcaccttt gggcgaattg tgggctgcta 121 ttgaatcaaa agaagcggaa aaaatagcac cgcttccgct gaagatactg gacagactga 181 ttttggcgga gttagataga ttcgctgatc aaatggccca tttttacgcg gactatcgtt 241 ttggcacatt gattgagacg tatctccaat ttcttcaaaa caatgtctcc acgtgctaca 301 ttcactatct tcgtgatcga ctctattgcg acccgatcaa ttcactggcg catgattcag 361 cacttttcac gttggacctc ttgggcagaa ctctgacttc aattatcgcg ccaatattgc 421 cgcatctttg cgttgagttt tactcccacc atccg //