LOCUS       EY190149                 395 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa01g09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to emb|CAB59358.1| translation elongation factor eEF-1
            alpha chain [Anisakis simplex], mRNA sequence.
ACCESSION   EY190149
VERSION     EY190149.1  GI:159492624
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 588
            PolyA tail found inside read...SR being truncated to 547
            Potential full insert read...vec-to-vec length: 532
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa ggtggaccgt cgttccggca agaaggtgga ggacaacccg aaggcactca
       61 agtccggaga cgccggcatc gtggagctgg tcccgaccaa gccgatgtgc gtggaggcgt
      121 tcaccgacta cgctcccctg ggacgcttcg ccgtccgcga catgagacag acggtggctg
      181 tcggagtgat caagggcgtc gagaagtcgg agggcggcgg aaaggtcacc aagtctgcac
      241 agaaggctgg cgccgctccg gccaagaaga agtaacgaat gaggacacgg tctttgaggg
      301 acgcaaagat tgcaccacca tctcttgaca ctctttcttt gttcatcgtt gtcctctatc
      361 atattgtcgt tacgttggat aagtttattt tgcca
//