LOCUS       EY190147                 309 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa01g07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190147
VERSION     EY190147.1  GI:159492622
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 160
            original value before phred recall for SR was 515
            PolyA tail found inside read...SR being truncated to 474
            Potential full insert read...vec-to-vec length: 446
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcaa ggatgcccgt agtaactgcg agtgaactgg gaagagtcca gcgctgaatc
       61 ttggtgcctc tggcgccaag agatgtagcg tacagactgc gagtttgggc tggcgcgtca
      121 gttcaagtct cccttgacag gggtccctca gaaggtgcaa ggcctgtcca acgggtgcag
      181 acagtccatt ttcgcgtgtc ttggagtcgg gttgtttggg atcgcagccc aaagaaggtg
      241 gtaaacttca tctaagactg aatatgacta cgagaccgat agcgaacaag tactgtgaag
      301 gaaagtcga
//