LOCUS EY190142 426 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa01f11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_610361.1| CG8707-PA [Drosophila melanogaster] gb|AAK92934.1| GH16429p [Drosophila melanogaster] gb|AAF59120.2| CG8707-PA [Drosophila melanogaster], mRNA sequence. ACCESSION EY190142 VERSION EY190142.1 GI:159492617 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 426) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 154 original value before phred recall for SR was 761 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..426 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcgt ccagaaggtt gacggactta gcgaggaaac gaaaatggag gcgcatcgga 61 agatattcca gactgtgcag gacgacttgg ccgaccaggg aatggaccac attcgggtga 121 attaccacat gacctcaatt tacgatcact ccatctttga ggcgttcagc aaagtgatcc 181 aaaaactgat caagcagctc tctgtgctgg agtcgttgct ggacattttc aatcagggtt 241 ccaacgtcga gaagtcattc ctgttcgatg tggccagcaa gatctacatt gccacggatt 301 cctcgcctgt agatatggcc acctacgaat tgtgctgcga catgatagat gtcaccattg 361 acatctcctc aatctacgga catcgcaacg agatgcaaca ccctcggcct gtgggcttcg 421 atcaag //