LOCUS EY190141 177 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa01f10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190141 VERSION EY190141.1 GI:159492616 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 177) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 777 was extended to value 777 (,) This trace has been recalled with phred original value before phred recall for SL was 155 original value before phred recall for SR was 377 PolyA tail found inside read...SR being truncated to 336 Potential full insert read...vec-to-vec length: 294 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..177 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa gtcgaagcgg ccgcagttgc gcctcaagta caaggagacg atggcacgcc 61 tgtacgagga gggcggcgag aacggcaccg gaagatgagc ggcggaggaa ggaaaaagct 121 caaacatctc acacttaaaa cgacaaaaca cagcgaataa actgaagcca attgtca //