LOCUS EY190137 338 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa01f06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190137 VERSION EY190137.1 GI:159492612 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 338) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 815 was extended to value 815 (,) This trace has been recalled with phred original value before phred recall for SL was 160 original value before phred recall for SR was 545 PolyA tail found inside read...SR being truncated to 505 Potential full insert read...vec-to-vec length: 476 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..338 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggca gaagggcgcc ggcaaacagc agtcggctgc caccgaaaat ggttggggtg 61 ttgctgagca gcctgtggct accaacgccg acgcggatgg atggggcaac gatcaaccgg 121 tggcggccaa agtcaacgca aatgggtgtg gcaaagagaa tgctgttgtg gctgtggcag 181 aggagcccac cgactacgga tgggagtgaa acggtgttga cgaaaagaaa gcagtttcta 241 tcgctgccaa tgatcccccg atatatactt attttggcat ttttgcagcg cattttttaa 301 aatgtatcac ttttttcgag taaatagcgc aattccca //