LOCUS       EY190132                 315 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa01f01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190132
VERSION     EY190132.1  GI:159492607
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 773 was extended to value 774 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 153
            original value before phred recall for SR was 508
            PolyA tail found inside read...SR being truncated to 473
            Potential full insert read...vec-to-vec length: 450
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcat cagaatgcag gagatgcagc agaaaatggg cacgcaaaga gtgagaaaca
       61 gcgcacgtcg acttggttct cctcaaaata gactgcgcag caatcgtctg gaccgcaccc
      121 gatccaacct ctcaacttgt tttctcgcta ctctgtgatt gtcttgcctg aactttgagc
      181 ggatagcggt ggatataaat tataaactga cagtaatcaa tcactactat atgcctgccg
      241 tgtcagtgta tcgtttcccg tttgtactct ggagattgca acaatttctt atcaacaagc
      301 aatttcctcg cccca
//