LOCUS EY190129 214 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa01e10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190129 VERSION EY190129.1 GI:159492604 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 214) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 773 was extended to value 773 (,) This trace has been recalled with phred original value before phred recall for SL was 155 original value before phred recall for SR was 778 PolyA tail found inside read...SR being truncated to 368 Potential full insert read...vec-to-vec length: 247 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..214 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcac cgggcgcaaa tggcccttac ggaccgttcg gagtggggtt ggccggcatt 61 ttgcagggcg tcatatttgg cggacgctga attcagagaa tgccactttt tgtgaatttt 121 gtggcggaaa cttggaagcc aaaacccaat aaattgactg ctcgattttc gaagcatggc 181 actttgaatt taatgcataa atttatttga atga //