LOCUS       EY190112                 400 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa01d04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to gb|AAK31158.1|AF348330_4 N utilization substance protein
            A [Wolbachia sp. wRi], mRNA sequence.
ACCESSION   EY190112
VERSION     EY190112.1  GI:159492587
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 811 was extended to value 812 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 158
            original value before phred recall for SR was 601
            PolyA tail found inside read...SR being truncated to 566
            Potential full insert read...vec-to-vec length: 539
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccaggtgg agaggtaagg tatggtattg ttaagcaagt aggatatcca gaccttattg
       61 ttgatataaa tggtaccact gcatttcttt cgttaaagaa tttaataggt gacgagagat
      121 ttcgtgaagg tgataagatt aaggcttata tacaaagtgt cagacgttct gatgatggac
      181 gacaagtcat tctatctcgt acacatccag gattcttaga agcattgtta aagcaagaag
      241 taccagaaat tactgatgga cttgtggtaa ttaaagctac agtgagagat gctggatcga
      301 gatcaaaagt tgctgttttc tctcctgata aaaatattga tccggttggg gcgtgtgttg
      361 gcaaaaagga aatgaaaaaa taagagaaaa tcaagaggca
//