LOCUS EY190108 231 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa01c11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190108 VERSION EY190108.1 GI:159492583 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 231) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 153 original value before phred recall for SR was 426 PolyA tail found inside read...SR being truncated to 385 Potential full insert read...vec-to-vec length: 262 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..231 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtcg accagctcca gtgtgcctga ttgggagcgg ggagaaggag aaaggcaaaa 61 aggagaaaag caaggaaaag aagaaacggg ccaagcgcat tggcagcgga ctgaagaacg 121 aggaagaaga agagtgctgt gaattcgaaa ttgtctcttg tgaccaaaag cgttgggagt 181 tcagtgctgt cttcggtgga cgagcgcgac aaatgggtgt tgtagcatcg a //