LOCUS EY190102 550 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa01c04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_446250.1| SAC1 (supressor of actin mutations 1, homolog)-like; suppressor of actin 1 [Rattus norvegicus] gb|AAG29810.1|AF251186_1 phosphoinositide phosphatase SAC1 [Rattus norvegicus], mRNA sequence. ACCESSION EY190102 VERSION EY190102.1 GI:159492577 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 550) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 805 was extended to value 805 (,) This trace has been recalled with phred original value before phred recall for SL was 152 original value before phred recall for SR was 807 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..550 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcga tttgcaccga cattgttctt cgcttaattg ggatcggctg gccatattga 61 agagcgaact agagccagat gtccgcaagt tcggtttctt ttgcacgaaa atcgagcgcc 121 aaagcgacgg acaattggtg gaggctagcc ctaaactgcg gcgaaaccag aatggcttct 181 ttcggacgaa ttgcatggat tgtttggatc gaacaaacgt agtccaagcc atgctcggcc 241 tggaatctct gcgactccag cttcagcata tcggcgtcat agaacattct acgcctaact 301 ttgatgcgtg gccggaattc attttcatct tcaaaaacct ttgggccgac aatggtgacg 361 actgcagcaa acagtatgcg ggcactccgg ctctaaagac ggactacacg cgaattggaa 421 agcgaacaat ggctggggct atggcggacc tgcagaactc actggcgcgc tatttcaaaa 481 acaatttcat ggatggttat cgccaagatg caatcaactt gttcctcgga aattgtgcga 541 tcaatggaaa //