LOCUS       EY190097                 511 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa01b10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_061401.1| 86 pct identical to 50 residues of 523
            aa protein gp:AB024946_31[hypothetical protein of plasmid pB171]
            [Plasmid F] dbj|BAA97892.1| 86 pct identical to 50 residues of 523
            aa protein gp:AB024946_31[hypothetical protein of plasmid pB1, mRNA
            sequence.
ACCESSION   EY190097
VERSION     EY190097.1  GI:159492572
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 153
            original value before phred recall for SR was 771
            COMM   possible reversed clone; protein similarity on positive
            strand
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc cagagtgaat aattgggttc gaagttttaa gtaattccgc gccaaataca
       61 ttttaatata atacttattt tgaactgaat ttgacctcaa aatgagtgaa agcggcgaag
      121 gaagcatcag caagcgggag aagaacaaat tggcacagac aaagcacaga gaggtaaaag
      181 tgttttggtc ttgattattt ctatatttct ttacttcccg caaagcagtt gttgcaaatg
      241 agcaaaaata gaatggaaca ggcatctgcc gacgggccgc gactatgcga tgtgtacccg
      301 agcaggatac gtccactggt cgcgaatcga acagcgatcc tgatagttga tgtgcagaac
      361 ctgatgacgg acgagggcag gtatccgctc agtccagagt tttacaaaga ggctagactg
      421 gccccctgaa tctccagaca accaatatca cttaaataag tgatagtcct taatactagt
      481 ttttagacta gtcattggag aacaaatgat t
//