LOCUS EY190090 196 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa01b02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190090 VERSION EY190090.1 GI:159492780 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 196) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 805 was extended to value 805 (,) This trace has been recalled with phred original value before phred recall for SL was 152 original value before phred recall for SR was 430 PolyA tail found inside read...SR being truncated to 350 Potential full insert read...vec-to-vec length: 338 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..196 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggggg cattggactc tgagacgtgg aagagaactg actgtccaca tttctattgg 61 ttagacaaga atgttgttac atttgtgcat tatgaacaga tacgacggcg aaatgttcaa 121 attttgtaat tattcacgta ttcttgcgct ttcttttttt tcgcttaaaa aatcaattgc 181 aaaaaaaaca aaaaca //