LOCUS EY190088 308 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa01a11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to pir|A88470 protein C28H8.3 [imported] - Caenorhabditis elegans, mRNA sequence. ACCESSION EY190088 VERSION EY190088.1 GI:159492778 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 308) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 755 was extended to value 756 (,) This trace has been recalled with phred original value before phred recall for SL was 152 original value before phred recall for SR was 507 PolyA tail found inside read...SR being truncated to 455 Potential full insert read...vec-to-vec length: 330 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..308 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggggg tatgaatttg ccctgtttac ggtcatcttc ggcatcgata ccccaaaact 61 cactccgctc caattccggc aaatgtccgg acgtgcaggc cgtcgtggct tcgatccagc 121 cggaaacgtg atcttctcat gtttgcctac gagcaagatt cggcgtctgc tcacggccag 181 tctggcaacg ctgcgaggca atgttccctt cacagccgca tttttgcttc gactgtttgc 241 ctatattaac ggcgagaaaa gtgggcagga taaaacggga gtgaacgaac aaaagggaaa 301 agctacca //