LOCUS       EY190061                 549 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa04f04.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to ref|NP_495781.1| Putative protein, nematode specific
            [Caenorhabditis elegans] pir|T21240 hypothetical protein F22B5.4 -
            Caenorhabditis elegans emb|CAA90356.1| Hypothetical protein F22B5.4
            [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY190061
VERSION     EY190061.1  GI:159492751
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 59
            original value before phred recall for SR was 633
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttctat aaagggtttt tttttttttt ttttctaact aggacccttt
       61 tgagggttac actacgacca gaaatgatgc cccaaaaaat gatcaatgaa tgggggatta
      121 gtctctggtt tttcgtttca tctttacatc tgctttgctg gccttcccgt tctagttagt
      181 aacaatgtag ccaccggcat cgcgggttcc acgctgcttg gccgtctcca acatctcatc
      241 ctgtagcttc tccatgattt ctatacgctg gtgggtcccg ccaagtggat cgaaaaatac
      301 tgtttgcatt ctccagtatt tgtcacgaat gcgcgcccaa tcccatggtg gggtcttggt
      361 gcttctgtca agccagactt ccactttttc aaaacttatg taggctatgc ccacgactag
      421 gcaacaagag acaccgcaga gaaatacgat cggccaaagc tccatgtgat ggcgagcgtt
      481 aaatgttaca aatcttataa atgtgtcggt cttcttatgt ggctttgtaa taacgtgatt
      541 cgcgtgaaa
//