LOCUS       EY190059                 402 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa04f01.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to gb|AAH41254.1| Similar to splicing factor 3a, subunit 2,
            66kDa [Xenopus laevis], mRNA sequence.
ACCESSION   EY190059
VERSION     EY190059.1  GI:159492749
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 829 was extended to value 830 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 55
            original value before phred recall for SR was 452
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgcccctg gggggttttt tttttttttt tttgaacgtt ggacgctttt
       61 taggggacaa gcaatagtaa aacacacatt catcgtctcg gtcgttagat catggattat
      121 aatcaatttt tcctggtccc cttaatcaac ttcacaatca caaactaata ttgtgttggg
      181 aggaacatag gtggcggagc aatgggcgga atctctacat ccccctgttg caaggccctg
      241 gcataatttg cgtcatcgtg gatatgtttg aatgcaaatt gcataaagta ctggcgactt
      301 tctttattcc acagagtcca gaatttgtct tccagcccat cgatttctct tgctggtatc
      361 ttaaatgcga ttgtttcata tggctctgct gccccggccg ta
//