LOCUS       EY190052                 587 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa04e05.b1 R.similis_EST_RSAA Radopholus similis cDNA 3',
            mRNA sequence.
ACCESSION   EY190052
VERSION     EY190052.1  GI:159492742
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 881 was extended to value 882 (,)
            putative full insert read: vector-to-vector length is 734 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 59
            original value before phred recall for SR was 793
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttctat tagggggttt tttttttttt ttttctcaat gcggaatctt
       61 ttttggaata atattaggcg aaataaaatg cggaatggaa ctgttaaatt caacagttat
      121 tactctctct tttcccattc gaatccaact cttgtattcg ctttgcaatg gcgtcacccc
      181 tctgccgcag cagctgagtc cgaaattcgc ctacttttat tagggtgcgg attgagtgaa
      241 tgtcgttgtc aattgcgaag aggctggcca tttgctttgc atttcggtcg gcgcacttct
      301 tcaggtcgtt cagatatgcc tccggaataa gatgccgttt tgaagtggcc acatcggggc
      361 gatagaattt taggtgcttt tcttcgcgaa agccttcttg tacttccggc tgctcccatc
      421 cgccgtcgtt ttttgcagtt cccggtggcg aattggattc ttctgactgt tctttgggtg
      481 tatcacttct tttggatggc gagcttgacg gatgttttga gcgcaaggct gaggcttttt
      541 tgttctacat tgatgctctg aagttgtggg gtcgacaacg agttggg
//