LOCUS EY190043 445 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa04d05.b1 R.similis_EST_RSAA Radopholus similis cDNA 3', mRNA sequence. ACCESSION EY190043 VERSION EY190043.1 GI:159492733 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 445) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 58 original value before phred recall for SR was 576 Seq primer: pDNR-LIB-reverse. FEATURES Location/Qualifiers source 1..445 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 tttttttttt tttaaaatga ggctcctttt aagggattcg tataaaatgt cggcaaacgc 61 aaaagaatat cacgatttga gtggcaaacc atttggtttt tttcaatgaa aacaggctaa 121 gttaggaaat ggcctatagc aattgtcgat ttggacggac gtcacacatc agggcaacac 181 gtcatcggcg ttcttcatgt tcgccacttc cggcatgtag ctcgagcgca tgtgcggcag 241 cgtcttctgg tttcgctctc gcaactcccg aaccctttcg tcgttattcg atccctcacc 301 ggtggttgag gatgaaggtc gcagcgatgg ctgatcaggg aagaatgtgg cgcggatgga 361 gggcgtcctg tcgatcttct tgcgagtacc gtttggtacc ggcattgcct tgggtcgttg 421 gaattcgacc gactcattct gccgg //