LOCUS       EY190041                 493 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa04d03.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to sp|P05086|EF2_MESAU Elongation factor 2 (EF-2)
            pir|A25440 translation elongation factor eEF-2 - Chinese hamster
            gb|AAA50387.1| elongation factor 2, mRNA sequence.
ACCESSION   EY190041
VERSION     EY190041.1  GI:159492731
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 56
            original value before phred recall for SR was 542
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgtccttt tgggggttct tttttttttt ttttaacgta tagattcctt
       61 tagggggtca gccaacaata agaacaaaga gtgtcgtgtg gcaaacatcc atttaagaat
      121 aacatcgtgt cttaaaggat aagcgatgaa cttgcttctc ttactccatc ttcggttttc
      181 tctttagagc ttgccgtaga agttgtccaa tgcggggatt ccctccttca gccctttgcg
      241 ttttcgggtc tcgacgacca cttgcgctgg acgcgagctg gcctccaagg gatctccggg
      301 cagaacctgc cagtggtcga acacgcattg cgggaatgcc tgtccgccgg tgttagaacg
      361 cagatcagcg gtgaaaccga aagactcgtt gacgggcaaa tgtgccttca ttatgaacac
      421 tggggtgcct gcgatttgac tttcctcaaa tacaacgcca cggcgacggt tcaccactcc
      481 gtacccggcc gta
//