LOCUS       EY190039                 452 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa04c12.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to ref|NP_498566.2| Putative nuclear protein of eukaryotic
            origin [Caenorhabditis elegans] sp|Q09385|YMWB_CAEEL Hypothetical
            protein K04G7.11 in chromosome III gb|AAA62538.3| Hypothetical
            protein K04G7.11 [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY190039
VERSION     EY190039.1  GI:159492729
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 56
            original value before phred recall for SR was 722
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga cagttcccct cagggggggt tttttttttt ttttgcgata caaagtctct
       61 ttgggggggg atcaaatatt ctcccggatt agacacaaaa ataggtaata tgtttgaact
      121 ttcttcgcat cctcttcaaa tggccgttcc tctctccaag tcctccttga tgtcttgcgt
      181 gtactttccg tagtagcgct ccaatttatg gttgaatttg cggttacgct cgttgatgaa
      241 atcgataggc gcgtccgggt cgaacatgcg acggcgatgg tactcgtcgc gcttcttcat
      301 ttgattgtgg acgtcctcgg acagcttgtc cagccccttc tgcgtcggat aatgggagcc
      361 tgtgatcagt gtgtttgcgc ccgcgtagaa ctgctcctcg cccacgacct ccttcatctt
      421 ctggtacgag tccatgtccg gcttgagcgc at
//