LOCUS       EY190035                 235 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa04c08.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to ref|NP_536715.2| ATPase, H+ transporting, lysosomal V0
            subunit A isoform 4; ATPase, H+ transporting, lysosomal (vacuolar
            proton pump) noncatalytic accessory protein 1B; V-ATPase alpha 4
            [Mus musculus] gb|AAN45855.1|AF435090_1 vacuolar proton translo,
            mRNA sequence.
ACCESSION   EY190035
VERSION     EY190035.1  GI:159492725
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 838 was extended to value 839 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 28
            original value before phred recall for SR was 324
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 gattcattca caacaagttg taaaaaatca gtatacgatc gattcggaac acaatttttc
       61 ttttgagttg acaaagctga acggggcgaa tgcatttcca cagccctcgt agaacttgct
      121 ttggaactca acccaatgca gacaaagggc gtgcaagaac gcggagagac cttccatcac
      181 gaccagaatc acaaccatca gtaagaagaa gacaaagaac gccaccccgg ccgta
//