LOCUS       EY190032                 559 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa04c04.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to pir|T46974 leucyl aminopeptidase (EC 3.4.11.-) ES-62
            precursor [validated] - nematode (Acanthocheilonema viteae)
            gb|AAC28365.1| aminopeptidase ES-62 precursor [Acanthocheilonema
            viteae], mRNA sequence.
ACCESSION   EY190032
VERSION     EY190032.1  GI:159492722
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 53
            original value before phred recall for SR was 607
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttcttt aagggggtct tttttttttt ttttgtgcta atgtttcctt
       61 ttgggggcag caatcattca caccaataat taatcgattc actaaatgct acatagttca
      121 catcggcact gttcaaaaat atccgtttgc cttccccgtt cagggcaatc atggccaatc
      181 gtccatgttc gcgattgtgt aggccagagt gccaaaaatg gccgctgtgt agtcgatgtc
      241 gtcctcctcg aaaatgttca ggtagtctgc actgctgtgg tggaactgga agtagaagtc
      301 gaagcctttt tcggcgcagt aattcaccga tggaacgccc agatctgacc agaaagtgac
      361 gtcgccctgg tcttttctcg ggacaacact cagggggatt cccaagaggt tcatcagctg
      421 gacgacttgc ttcaccttgc ccatctgtcg ctcggatccg gcgaatttga gcatcgactt
      481 gtaggtcctc ggccggaagg cgccctggtc tgtttcggag acgaacacaa acctttcatc
      541 ggagtccttc ccggccgta
//