LOCUS       EY190029                 287 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa04b12.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to emb|CAA70477.2| SEC-2 protein [Globodera pallida], mRNA
            sequence.
ACCESSION   EY190029
VERSION     EY190029.1  GI:159492719
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 50
            original value before phred recall for SR was 333
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttctat agagggtttt tttttttttt ttttggaagt tggttccttt
       61 ttgggggcaa ctgatactga tttgatttgt ttctcttcgt tctctcaatg ttcagttcct
      121 tccttcatct tctgcttagg cggcggtggc ctccggcttc agcagactct gggccaactt
      181 ctggaacttc tcgttttgga tgacgccggt gatcttgggg aaattgctct tcaaggactc
      241 cttcgcctcc tcgctcagtg ccttgtactt ctcgatgccc ggccgta
//