LOCUS       EY190020                 262 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa04b02.b1 R.similis_EST_RSAA Radopholus similis cDNA 3',
            mRNA sequence.
ACCESSION   EY190020
VERSION     EY190020.1  GI:159492710
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 537 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 53
            original value before phred recall for SR was 590
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ttttatgtta ttattactta aataaagcgg tcatttctgc tggggctgct gctgtttttc
       61 gacaccattt ctgtttgctc tcctccatcc ccccttcagc aatgccgaca gactgccact
      121 gctccaacta cctttttgca gcttttcgtt tctctccttc gacccccctt tcccctcctc
      181 cacgtttcgc tcttcagttt gcggcatttt tggcaccttt tcctgctcct ccagctgacc
      241 catcaatatc gacatctgca ag
//