LOCUS       EY190016                 543 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa04a08.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to ref|NP_500197.1| Sec61beta family (8.6 kD)
            [Caenorhabditis elegans] gb|AAK68450.1|AC025715_12 Hypothetical
            protein Y38F2AR.9 [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY190016
VERSION     EY190016.1  GI:159492706
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 538 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 57
            original value before phred recall for SR was 595
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttcaat aaggggtctt tttttttttt ttttgaataa agggcccctt
       61 tttgggggag agctttagat tgccaggata tcacttggat cacttgttta agaaagtaaa
      121 caaattgtgt ttacaggcgg gtggggggat ttgtccattt cttgtggtgt tctagcttca
      181 gtgaccgcga ctgtattttc cccagatgtg aagaatgaaa acgctggcga tgaacacaag
      241 tgacatgacg agcacaggga ccggtccaat cttcagtcca gtcgagtctt ccgtgtagaa
      301 gcgccatagt cctccactgt tcattccgcc accgcctcca ccgccgccgg tagtgcgagt
      361 tctgttgttg gtcgtaccgg caccacgccg ttgtctgagt gctccaccag cagccgtgcg
      421 aggaactacc ttcccgccgc caggtacttt tgctgagtca gccatagtaa ctatacgtaa
      481 aatttcactg gtaattccta ataaatttta aaatatgact gaattgtttg tgtccccggc
      541 cgt
//