LOCUS       EY190004                 331 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03h06.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to gb|AAG31480.1| ubiquitin-like protein [Wuchereria
            bancrofti], mRNA sequence.
ACCESSION   EY190004
VERSION     EY190004.1  GI:159492694
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 848 was extended to value 848 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 58
            original value before phred recall for SR was 559
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ccaatttata cgtcaacagg ggtgaacgat taacagctca aaagggcgat ggacatcttt
       61 tgctcttccg ccaatccatc tgcttcttca cttaagtttc ttcttgacgc ggaggtcgtt
      121 ggagtgaccg cacttcttct ttcggcagtt ggttgcacgc ggaggcaaac aagcatagca
      181 cttgcggcaa atctgcttgt cacagttgta cttctgagcc aacatgcgca gcgacggctc
      241 aatgattcct ccgcggagac gcagcaccaa gtgcaaagtg gactccttct gaatgttgta
      301 atcccccaga gtacgtccgt cctccaactg t
//