LOCUS       EY190003                 546 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03h05.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to ref|NP_504684.1| Predicted CDS, palmitoyl protein
            thioesterase PPT-1 (ppt-1) [Caenorhabditis elegans] pir|T30106
            hypothetical protein F44C4.5 - Caenorhabditis elegans
            gb|AAA92329.1| Palmitoyl protein thioesterase protein 1
            [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY190003
VERSION     EY190003.1  GI:159492693
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 48
            original value before phred recall for SR was 588
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttcagt cagggttttt tttttttttt ttttcgaatt tgggacactt
       61 tggggggtcg gaatgatgat gattttcttc tctttaatca tttatacatt ttcccgcagc
      121 tcttgtccga gcacattcct tcctctcctt tgacgttgaa actacctcct tcctctccct
      181 tccccagccc tttttgatca ccacctctgc cggaatttgg aggtgtgctc cgtcgatggc
      241 gagaaagtgc agtctgcgcg agtcgtcgag cgccttcagc ccaacgcggt ctccccgcga
      301 gtagagcgca gtctcctcca tcggttccac agtgccggtg ttgttttcag catagaatcc
      361 gaaccacgag gactccttgg gctgaatcat ctgatcgcgc aggaacttga caagcaaaaa
      421 gttccgcagc tttagcagat ttgtgcggta agttgcgttc aaggtcagtt cgcagttcag
      481 atcggccaga aagatgttct tgtgtcggta gtcttcggcc aggttcggat cgtgcccccg
      541 gccgta
//