LOCUS       EY190001                 657 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03h03.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to ref|NP_491243.1| Guanylate kinase (24.9 kD)
            [Caenorhabditis elegans] gb|AAB42236.2| Hypothetical protein
            T03F1.8 [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY190001
VERSION     EY190001.1  GI:159492691
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 54
            original value before phred recall for SR was 850
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgtcccta caaggttttt tttttttttt ttttgatttt ttgaagcctt
       61 tagggggctc cataagctcg gctgtgtcta caacacgtca aaaatatcac tttccgacca
      121 gtcattccga atttggaaca gcgtccaaac aaattgcacg aatgatcttg agtcttttcc
      181 tatggggaat attgacaata gaatgggaag agagctcgca atatgtcgaa gcaatagcca
      241 gtgaaagaca actactattt atgtatagaa tctaaaaatc taaatataga attgaagaag
      301 caacagaaga gcggaatgga cttttattgc gtgtgcctgc cacagcactg ttcttgttcc
      361 ctcctagtca tcttgaactt tacttatcgc cgtctacgga ctgtagtcgt tgttgcagtg
      421 tgtccagttc aggtcgaatt acatccacga attctccata ggccttgtcc agaatgtcat
      481 tcacgatgat gtagtcgaat agtgatggct gctgctcaac cgcttccaaa tcttctttcg
      541 cgtggttgag ccgtttggtg atggagtcct cagtttcggt gccacgctga cggagccgtt
      601 gttccaggac ttcaatggat ggcgggcgaa caagtatgaa tttcgcgtca aaattgg
//