LOCUS       EY190000                 628 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03h02.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to pir|T31808 hypothetical protein M03F8.3 - Caenorhabditis
            elegans, mRNA sequence.
ACCESSION   EY190000
VERSION     EY190000.1  GI:159492690
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 793 was extended to value 794 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 35
            original value before phred recall for SR was 731
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgctcttc actgggtttt tttttttttt tttcgctttt tacggtcttt
       61 taggggttca ttcaattcat attggaaaaa acatggttta taagaaaaat tatgattcca
      121 aaggaactgc ggattcgacg gctgccgcat ttttctcgcg ccaaagtcgc gctgcttcca
      181 gcaatttgaa gttcatcttg gatgtttggt cttgcgggaa aatgtaatcg aagtactcct
      241 cccagcccgc gtctgttcca tcagttgccg taatctgtcg gcgtttctta acgcgttttg
      301 gcatcatttt ttgtacggaa ttcagctccg cttcatttcc acgtttctgt tcaaattcat
      361 tccattgttc gagcagcagg agtctttctt cggctagcgc cccttccagt gcctggtttg
      421 cgcgtttgta gattgccctt gcccgctcta tttcgtcgtt gctcgtctcc cattcgatcc
      481 agctgatcca cacctttagg tgctgtgtcc gtcgaagcag actctcgtag agaatacgaa
      541 ctctgtcgtg ctctccctgt tcaatctcaa agtcgatgta cgccttccag agcacttctg
      601 gcatgtccaa tgacggctgc tgaatagc
//