LOCUS       EY189996                 425 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03g05.b1 R.similis_EST_RSAA Radopholus similis cDNA 3',
            mRNA sequence.
ACCESSION   EY189996
VERSION     EY189996.1  GI:159492686
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 851 was extended to value 852 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 52
            original value before phred recall for SR was 474
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttcttt ttagggtttt tttttttttt ttttgtgata tttcatcctt
       61 ttagggttct cttctcataa tcatgtttca tgtgtgtgga aggaagaaca aagagattgg
      121 tgatttacaa tttacataca tatcatgctc tgttatcttt tacttacatt caaaagagag
      181 aggccgattc aaattttcgc atttatgcga ccaagccttg agaagaaatg tgtgtgtttg
      241 gaagaacaaa aaaataagag tacacgcgct cagaccgaat aatatgtgtg tatgttgtgg
      301 agcaaaacag agagaaagaa tcaaagaaat gattgaatca aatgcaacgg cacatccctt
      361 tcgcagttaa cagcatcccc ctctgaacag agaattagcc gcactctagt cctcgcccgg
      421 ccgta
//