LOCUS       EY189993                 425 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03g01.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to pir|T15098 hypothetical protein T22B11.5 -
            Caenorhabditis elegans, mRNA sequence.
ACCESSION   EY189993
VERSION     EY189993.1  GI:159492683
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 54
            original value before phred recall for SR was 473
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgtccctc aaggggtttt tttttttttt tttggtatgc aaggctcctt
       61 atagggaaca tggaactaga tcactttgac aactggatgg taaatatgat aaacgagtaa
      121 agcttgattc ctaatcgtca ttcaaatcat ccaatctcaa ttcaccggtt tcgccaaaat
      181 tgcgtccaac attccggtct gctccttctt gtgcatgaac ttgttgccgg tggcaggcga
      241 tgcactaggc ttgcgtcccg cgtacaaaag tccccgattg ttcagcttca ggaaggagag
      301 gaaacgcggt tgcacgaatc cccacgcgcc catattcttg tgttcttcct gcgcccaaat
      361 gagctcggca ttcgcgtagg tgttgcactc ggtgatcagt aaatcatacg ggaatcccgg
      421 ccgta
//