LOCUS       EY189990                 513 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03f10.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to ref|NP_505708.1| Putative protein family member of
            bilaterial origin [Caenorhabditis elegans] sp|Q19978|YV6L_CAEEL
            Hypothetical protein F32G8.4 in chromosome V pir|T21670
            hypothetical protein F32G8.4 - Caenorhabditis elegans
            emb|CAA96648.2| Hypot, mRNA sequence.
ACCESSION   EY189990
VERSION     EY189990.1  GI:159492680
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 58
            original value before phred recall for SR was 721
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgtccatt gaggggtttt tttttttttt tttgcgctgt agcatccata
       61 aaaaggtgct cagttttttc ctttgttttg cttccaactc tgccatagcg cctggcaact
      121 catgtgcgtt tatcaactgc atctctttgg gtggaatttg gaagtcggcc aaatgccctt
      181 tcggaattat gtcctgtatt ttgaaggaag cgcgcgtgtg tggcaatgtc acttccatct
      241 cgtcgctgat tttcgacggc atttgatcaa ccaattcgga cgccatcata tgcgctatgt
      301 acgtgttcct gtcgagcaca tagttcagtt caaagtcgtc gtcctcctct ccgaacggat
      361 tcatgaagaa ctgtccgact ttgagccatc cgatgtaaaa gaacatttgg aaggtggtga
      421 atatgggaaa caaaatggtg atctcctgct ccattgactt ggcgtccagc ttctgttgtc
      481 gccccatcaa acagatgaag aaatatccgt agg
//