LOCUS       EY189985                 530 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03f05.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to ref|NP_500176.1| Predicted CDS, putative membrane
            protein, with at least 3 transmembrane domains, of bilaterial
            origin [Caenorhabditis elegans] gb|AAF60870.1| Hypothetical protein
            Y71G10AR.1 [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY189985
VERSION     EY189985.1  GI:159492675
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 863 was extended to value 863 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 47
            original value before phred recall for SR was 575
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttcttt ttgggggttt tttttttttt ttttatccta aaggtttctt
       61 ggggggttcc tttccttatg aaatcagaaa ttacaatact actaatagaa ttgaagatga
      121 gattttttaa tttcttcaac attaaaaatc tatgcgtctt ctgggtccaa tctcttttgc
      181 gcaaattcga taagttcgct tccggcatct ggcattctaa ccgacacatc gtttatgtcg
      241 actcttcttt tggccttgaa tcttttaata gtcgaatgaa cagcgtagcc gagcagcaat
      301 gggccgggtg tgaaacatgc gactgcgatt ggtaaaagac ttgcaatgtc ggactgcgtg
      361 ccagggaaag agagctttaa tagcatgatg gcaattcccg tattttgcac gccagtttct
      421 atggctatcg ccgtaacatc cggtggtcgt tgtgctaaca gaatcgacgt gaaacatccg
      481 aacatgaatc cgcaccaagg cagaagtagc cctgcaaata cccggccgta
//