LOCUS       EY189974                 534 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03e05.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to ref|NP_498586.1| Phophoribosylpyrophosphate synthetase
            II (41.1 kD) [Caenorhabditis elegans] pir|T16763 hypothetical
            protein R151.2 - Caenorhabditis elegans gb|AAK29853.1| Hypothetical
            protein R151.2a [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY189974
VERSION     EY189974.1  GI:159492664
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 58
            original value before phred recall for SR was 905
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga acatgtcgat cagggggccc tttttttttt tttttaaaat tgagaaccct
       61 ttaaagggaa aattatatgt ggtattgaaa aatgcaacta tattaagtta gttgagcgca
      121 caacgatgat ctttttgaac agcacaccct tcttcgtcat cggttggaga tcgcgaaccc
      181 cgccatttat tagagtggga catgtgagaa aaggtagcta acggactctc cattgtgtgt
      241 acgacggatt gcctcggcaa gaatcatgga aatgtcgatg cactgaattt tgttgcatcg
      301 tctcatattt tcctcctgag gaatcgtgtt cgtgacgaca actgcctcaa acttggattt
      361 gttcagacgg tcaattgcgg gcccagaaaa tatcccatga acgcaaattg cgtagatctt
      421 ctgcgcccca gcttcatata gcttctccgc agccagaaca attgtaccgc aggtgtcagc
      481 catatcatcg accagaattg cgatcttgtc ccgcacatct ccgaccaaag tcat
//