LOCUS       EY189971                 498 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03e02.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to ref|NP_491282.1| Calponin homology (CH) domain
            containing protein family member [Caenorhabditis elegans]
            pir|T29467 hypothetical protein F28H1.2 - Caenorhabditis elegans
            gb|AAB52338.1| Calponin protein 3 [Caenorhabditis elegans], mRNA
            sequence.
ACCESSION   EY189971
VERSION     EY189971.1  GI:159492661
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 54
            original value before phred recall for SR was 720
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga ctgttcaata aaggggcctt tttttttttt tttcctcttt atgttccttt
       61 tagggttttt ttttttcttg aattggccat ttattccggc atcgctattt tcaggggccc
      121 acagtttttt aggagagaga ggggaaaaca gttaacccct cggtattgtt gcaaggtcgc
      181 acacggggag aaaattccca aaacggggag ttctcgaatg ggagcgatag cacatgctct
      241 gtccgtcttt tgctgcactc acttctactc ttcatccaaa aaatgggttc ggctttccct
      301 tcttccccat aattcggccc aacgacaaca gggtcatgca cacggagtaa aggtcacgtg
      361 ccccaaacaa gtccaccctc tggaaggtct cctcgggggg cactccctgt ctcttcccca
      421 accacacgaa agcgttgatg ttctccatgc aggcaaagtt gccagccggc tgctggatct
      481 tcttgacgga ccccccct
//