LOCUS       EY189966                 343 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03d08.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to ref|NP_498657.1| Transthyretin-like family member
            precursor family member [Caenorhabditis elegans]
            gb|AAK84467.1|AC006679_8 Hypothetical protein R13A5.6
            [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY189966
VERSION     EY189966.1  GI:159491805
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 568 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 58
            original value before phred recall for SR was 626
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 atatggcgca aaaggcgtat tggtggggaa gcctagatca gcgggggtcc gcactccctt
       61 ttagttgatg caactgcgat cctcgtcggg aaacttgaac tccatgttca tagtgcccgc
      121 ctcgaaccac ttgctgactc tgtccgcccg ctgcacatac tggtccggga ctccgaggtc
      181 gactttgcgc tggcacggct tgactccgtc gtcgcagtcg cggtacacct tgaagtggac
      241 gctcatcttg gccagctggc cgaccccgcc ctgcagctcg aagttgccgt tgttgtcggt
      301 cttgccagcc gccagctggt cgtcggtgcc aagcgtgttc ttg
//