LOCUS       EY189963                 476 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa03d03.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to ref|NP_502655.1| Ribosomal Protein, Large subunit RPL-18
            (21.0 kD) (rpl-18) [Caenorhabditis elegans] pir|T26939 hypothetical
            protein Y45F10D.12 - Caenorhabditis elegans emb|CAA16387.1| C.
            elegans RPL-18 protein (corresponding sequence Y45F10D.12), mRNA
            sequence.
ACCESSION   EY189963
VERSION     EY189963.1  GI:159491802
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 54
            original value before phred recall for SR was 527
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttccat aggggggttt tttttttttt ttttatccgt tgggtcccca
       61 taagggggat gtaaagtccg ttgtctcctc tcgaagatat ttgctcctct ccaaccgcaa
      121 atgacttctt cttcaggtct tgtagccacg cgactttcgg cgaccacgag cgcgttcgaa
      181 ctttcttcct ttggcgcgaa ccaatggctt ggcatggctg ccaggtacac caggcggcgg
      241 tccgaaatgc ttctccgctt cgcgtgcctt gcgcttgccc tgcaagagca gcgtgttctc
      301 gcccttcggt gcgcgcagcg ccaattggtc caatgtgatg gtctctccac ccgcagcgat
      361 gatgcgagca cgcgcagagc tggtgaagcg cagagcgcag atagtcatgg ccgggacttt
      421 gtagatgcgc ttgtcgtcgg tgatagcgcc cacacaaact acaatccccg gccgta
//