LOCUS       EY189927                 495 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02h08.b1 R.similis_EST_RSAA Radopholus similis cDNA 3',
            mRNA sequence.
ACCESSION   EY189927
VERSION     EY189927.1  GI:159491958
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 590 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 55
            original value before phred recall for SR was 645
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttcaat gagggggcct tttttttttt ttttcctttt ttggtctctt
       61 ttagggtttt tttttttcat acgggatatt ttatatgcaa aacttcagtt acatggtcat
      121 gacaagtttt tttttaagtt gaaattattg ggtttgaatc ggatgattta acgtatcaaa
      181 aaggattttc tgagttcttg caatcttttc cccgcgtcta aaaccccccc ctcctggagc
      241 gttacgtctt gttccgggat catgggtatc tgtacaaggg gttgcgtgaa tttcattgca
      301 tcaaagggat tcccttcaag ctgctttcgg ggcgcatgct gaccagttac ttcggttccg
      361 ttttgttcaa atgcgtgcaa tgcggacaaa aatgtcattg tcccaatgtc cccatgcgtt
      421 attgggggag ccatgatttc gatctttgct gggagttcga tttgtcgggg aatgttgaga
      481 tctattttcc gagaa
//