LOCUS       EY189921                 486 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02g12.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to sp|P34388|YLS3_CAEEL Hypothetical protein F09G8.3 in
            chromosome III pir|S44795 F09G8.3 protein - Caenorhabditis elegans,
            mRNA sequence.
ACCESSION   EY189921
VERSION     EY189921.1  GI:159491952
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 50
            original value before phred recall for SR was 558
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgtccttt tgggggtttt tttttttttt tttggcactg aacgaatttt
       61 tagttgcttg aataaaaaca agaaaaaaca atccgttgtc cgtgtcaatc aacgcttttt
      121 ccaaatccat tttgctcttg cgccaggctg attgatcttt gaacgttcct tctttcttgg
      181 atcgcgggtc aaaagtccag ccaagcgcaa tttttcagtc cagactgggt aaagcgccgc
      241 caaacataaa gatgtcccat gtcttacgat tcggggtatt acagactgac caccgggtcc
      301 ttccttgatg cttgcctcta cgtccacttt ccccagcatt ccagtgatta tcattggggt
      361 cagcaagatt tctctagcct gcaaagaacg gaactcttcc aacccatgtc catccaccaa
      421 taattcccca actccttgat ccgtgattct aaccgtcatt ctagtcgctt ttaacgagct
      481 gacata
//