LOCUS       EY189916                 517 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02g07.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to ref|NP_492457.1| Translation elongation factor 2 EFT-2
            (94.8 kD) (eft-2) [Caenorhabditis elegans] sp|P29691|EF2_CAEEL
            Elongation factor 2 (EF-2) pir|T21362 hypothetical protein F25H5.4
            - Caenorhabditis elegans emb|CAB02985.1| C. elegans EFT-2 pro, mRNA
            sequence.
ACCESSION   EY189916
VERSION     EY189916.1  GI:159491947
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 194
            original value before phred recall for SR was 601
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgtcccct aaggggtttt tttttttttt tttttgccga ctgtgtcctt
       61 tgaggggtgg atgcgtgcct tcattccagt ggcaactttg cctgcgaaaa cacgggcaaa
      121 agcgtagaat cttcctttgt cagaagtagg caccatcttc gagatgtaca tcatcagagg
      181 gccatttgga tcgcagttct tcatcgccac ggccgcttcg tcgtcgtgtg gtccttcata
      241 gagcatttcc atgcggtact tttgagccgt tacgggagac ggcaagtgta tgcaaatcat
      301 ctgaagcatg gtgtccccag caggcagcca tttgcgcatc atggtcttca acagtggttt
      361 tccttcttgg tccttctctt catgagtcaa tttaatcccg agcttatcaa ctagcttggc
      421 gcattcctcc ttcttaatgt tcatcaccgc gtcgaacact ttgaaaatag gatccaacac
      481 gaattgacaa aaaccacgct ttgtttccgg ttcctgt
//