LOCUS       EY189914                 546 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02g05.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to ref|NP_510634.1| Ndr family (35.6 kD) [Caenorhabditis
            elegans] sp|O02485|YDJ1_CAEEL Hypothetical 35.6 kDa protein
            ZK1073.1 in chromosome X pir|T27688 hypothetical protein ZK1073.1 -
            Caenorhabditis elegans emb|CAA92227.1| Hypothetical protein ZK10,
            mRNA sequence.
ACCESSION   EY189914
VERSION     EY189914.1  GI:159491945
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 58
            original value before phred recall for SR was 635
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttcttt ttgggttttt tttttttttt ttttgtttgt tacatttctt
       61 taaattccaa atacgtatgc agttgcgagg gaccggtaac tggtgggtcc aaataaaagc
      121 tcttagctta ttaaatgtgt acatcaactc gattggcttc cacgacctgt ttccctcgcc
      181 gtgtctatgc cgtcgcgttg atcacgttgt tcatcagatt ctgcgcactc tcgtcaacct
      241 tcatcagctt ctgcgcattc tcgtccaccg acgtcttctt ggacattgtc gtgaagcgac
      301 ctgtcgggcg atctgcctcc tccatgctca agctctttgt gcgcattctg cccagaccag
      361 gcatgtccac cgcagccagc tcaccgaagc ccttgcagaa caggatcaga gaacgcgcaa
      421 gacgacttgg cgcctctacg agaacgtcca gcacatcgtc gatcatgatc aagttctttt
      481 cgcccccgcg cattttctcg tgcatgtggc ggacatgatc gttgtgcgga aagcgtgccc
      541 cggtga
//