LOCUS       EY189901                 416 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02f02.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to gb|AAO43307.1| putative polyubiquitin [Arabidopsis
            thaliana], mRNA sequence.
ACCESSION   EY189901
VERSION     EY189901.1  GI:159491932
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 865 was extended to value 866 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 56
            original value before phred recall for SR was 469
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgtccttt tttggggttt tttttttttt ttttttgcca ttttgtcttt
       61 tttactgttc cgcgaaagcc atttcagaat tacatatcga gaaagcatga ttacttaatg
      121 aatgtaggtt tttttctgct gttatgtata tcgtaaaaca tgtggattgt gttttgtgtg
      181 atcagaacag gaattaaaat tggtccacat ctcaagacgc catattcctc tgcgcgttct
      241 ctgactcagg cggaaccacc gcgaagacgc aacaccagat ggagggtgga ttccttctgg
      301 atgttgtagt ccgcaagggt gcgaccgtcc tcgagctgct ttccggcgaa gatcagacgc
      361 tgctggtccg gcggaatgcc ttccttgtcc tggatcttgg ccttgacccg gccgta
//