LOCUS       EY189899                 324 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02e11.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to ref|NP_497500.1| Phosphatidylinositol-4-phosphate
            5-kinase type II (45.5 kD) [Caenorhabditis elegans] gb|AAK29922.1|
            Hypothetical protein Y48G9A.8 [Caenorhabditis elegans], mRNA
            sequence.
ACCESSION   EY189899
VERSION     EY189899.1  GI:159491930
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 55
            original value before phred recall for SR was 499
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 tttgattttt tgattttgat gcaaatggag aaagctctat tctagggatc agtattcatt
       61 cttaactgcg tttgttttca tcagtgaata gcaatttgtg ctacttcaga cggaattgta
      121 tcttctacaa gtgtagccga aaccacatta cgattcacga attccagaag ccgtttcgca
      181 tactgctccg gtttaacggt agaaatttct cgctcagctc ctgagcccct tagggcagat
      241 tttgcggcgg attcggtcct cttcttcact ccataataag tcaaaatgtc gaccagacca
      301 atgaaataga ccagttttct cttt
//