LOCUS       EY189893                 588 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02e05.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to ref|NP_741143.1| enoyl-CoA hydratase isomerase family
            (42.7 kD) [Caenorhabditis elegans] pir|T16010 hypothetical protein
            F09F7.4 - Caenorhabditis elegans gb|AAA50696.1| Hypothetical
            protein F09F7.4a [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY189893
VERSION     EY189893.1  GI:159491924
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 768 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 61
            original value before phred recall for SR was 829
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttctct aaagggtttt tttttttttt ttttaatcag aagaactctt
       61 ttcggaaata gccaattcaa cagctaagcc atattagaaa taagtgaaaa ttggggaaaa
      121 tggcactcga ttacacattc catacaccaa gcgaataaat atcccttccg tttctggctc
      181 ttaactttcc tccagccgca attctcgcgt tggatcggca aacggtgcaa aataggcgtc
      241 cacagcagct tcgctaactt ccgccagtga ggctggccgc cacttgggct ttcggtcctt
      301 gttaatcaga attgccctgc agccctcgtg gaagtcgtgt gcggccacga agcgctgggt
      361 gaagcggaac tcgagtggga agacgtcgcg gaaagcgagc ttagctccct cgcgaatttg
      421 gcgaaacgcg actttgagcg aagtcgggct cattttcttc aaatgttcaa gttgttcgcg
      481 cgcaaaatcg ctgtcttctt tctcggcgct ttcctccaat ctggccatca ccgtctccgc
      541 actgtccgca tcgaagcagg cgtcaatcag caccaggtgc gggtcgag
//