LOCUS EY189876 303 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa02c09.b1 R.similis_EST_RSAA Radopholus similis cDNA 3', mRNA sequence. ACCESSION EY189876 VERSION EY189876.1 GI:159491907 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 303) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 58 original value before phred recall for SR was 681 Seq primer: pDNR-LIB-reverse. FEATURES Location/Qualifiers source 1..303 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 aaagcattgg tccacgggtg tctagtctgg gcagtaaaaa ttgggatgcc tgtgcttttc 61 ctcagagggt agttttcgaa cataatattg ataattgatt ggatcgacca taacaaaaat 121 agaagcatgg agaatgaatg gtatgcgacc acaagggctg tgagtcctgc gttagaatgc 181 cgcacacctc agtcgttctt gttccgaccg cggttggaag aattggtcca cgaaataacg 241 cgagtagagt cctccaggat gttctcatcc tggccgtaat tctgcgagct gccagtgccc 301 tcc //