LOCUS       EY189872                 605 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa02c01.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to ref|NP_500626.2| Metallo beta-lactamase/ thiolesterase
            superfamily member (93.9 kD) [Caenorhabditis elegans]
            gb|AAB92042.3| Hypothetical protein E04A4.4 [Caenorhabditis
            elegans], mRNA sequence.
ACCESSION   EY189872
VERSION     EY189872.1  GI:159491903
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 848 was extended to value 848 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 51
            original value before phred recall for SR was 853
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga atgttccttt ttggggtttt tttttttttt tcaaccatta atcgatcttt
       61 tgaaaattta caccggatgt caaaaaccaa caaacaagat ttaactttaa tcattcggga
      121 gaaaactttc ttgagcaaat tgttaggatc gctttgcgat caaattttca acaactcctc
      181 tttgaaaagt gagtgataga gggggagcag atgcggaaac acaggaagca tgtcgaacgg
      241 gaccaccatg ttgtccattg ctacgctgaa gttgcccgcc tggatcaaat agtcggggag
      301 aggcggcact cgtgaatacc ttgagctgaa gtgcgtgaaa atcgtgtttt tggctcgcat
      361 ccgatgtgcg gagtcgaatg cctgttgcat ggtgctgtgc cgtttgaatt cagcgtccct
      421 ctccattccg tcctcgaacg tgcactcgtg gaccaataca tccgcatcac gaccgtactg
      481 ctccaacaac tcgcagggct tcgtatcccc agaaaagacg attctgcgtc cgtccttttc
      541 acactcaaag atgaagccgt tcgcctgccg tgtgtgaatc acttggactg ctcgcactga
      601 ccgca
//